Enhanced Antimicrobials Production by Streptomyces sundarbansensis sp. nov. in a Novel Extended Surface Biofilm Reactor
نویسنده
چکیده
A newly designed extended surface biofilm reactor (ESBR) with an inner configuration consisting of eight equidistantly spaced polymethylmethacrylate rectangular strips placed radially on a circular disk to provide additional surface area for microbial growth was used for antimicrobials production by the biofilm-forming marine isolate Streptomyces sundarbansensis sp nov.. Antimicrobials production by Streptomyces sundarbansensis sp nov. is studied in small scale polymethylmethacrylate conico-cylindrical flask (PMMA-CCF), as well as in a 4.2 L ESBR. The best pH and temperature for antimicrobials production are determined (pH 10, 30C) through a designed experimental study using PMMA-CCF. Consequently, operating conditions in the ESBR are studied using a 3 experimental design wherein each of two (n = 2) parameters viz. aeration and inner configuration submergence are considered at three levels viz. high, medium, and low: 0.75, 0.50, and 0.25 L/min for aeration rate; and 75, 50, and 25 % for inner configuration submergence, (while maintaining the rotational speed at 140rpm). The 50% submergence condition together with maximum permissible aeration is found to be most favorable for antimicrobials production peak antimicrobial activity (PAMA) attaining its highest value, 47 mm. PAMA increases with increasing aeration at all operating conditions studied particularly, at 50% submergence condition, a threefold increase in aeration rate (0.25~0.75 L/min) causes PAMA to increase by 74%, thus showing the strong aeration dependence of this antimicrobials producer. Again, compare to the best values obtained in the 420 mL PMMA-CCF experiments, corresponding ESBR value is 15% higher for PAMA – strong evidence for applying this novel bioreactor for cultivation of antimicrobial-producing marine microbes.
منابع مشابه
Enhanced Antimicrobials and Esterase Production Associated to Biofilm Formation by Two Estuarine Isolates in a Novel Polymethylmethacrylate Conico- Cylindrical Flask
A newly designed polymethylmethacrylate (PMMA) conico-cylindrical flask (CCF) with an inner arrangement consisting of eight equidistantly spaced rectangular strips mounted radially on a circular disk to provide additional surface area for microbial attachment was employed for antimicrobial compounds and esterase production by two biofilm-forming marine isolates, Streptomyces sundarbansensis sp ...
متن کاملOcta Journal of Biosciences Optimization Of Culture Conditions For Enhanced Production Of Antimalarial Artemisinin By Streptomyces Sp. Using Response Surface Methodology
Received 02 Sept 2016 Revised 10 Oct 2016 Accepted 07 Nov 2016 Available online 03 Dec 2016
متن کاملLactic Acid production in a mixed-culture biofilm reactor.
Novel solid supports, consisting of polypropylene blended with various agricultural materials (pp composite), were evaluated as supports for pure- and mixed-culture continuous lactic acid fermentations in biofilm reactors. Streptomyces viridosporus T7A (ATCC 39115) was used to form a biofilm, and Lactobacillus casei subsp. rhamnosus (ATCC 11443) was used for lactic acid production. For mixed-cu...
متن کاملImprovement of Methanol Synthesis Process by using a Novel Sorption-Enhanced Fluidized-bed Reactor, Part II: Multiobjective Optimization and Decision-making Method
In the first part (Part I) of this study, a novel fluidized bed reactor was modeled mathematically for methanol synthesis in the presence of in-situ water adsorbent named Sorption Enhanced Fluidized-bed Reactor (SE-FMR) is modeled, mathematically. Here, the non-dominated sorting genetic algorithm-II (NSGA-II) is applied for multi-objective optimization of this configuration. Inlet temperature o...
متن کاملStudies on the Influence of Various Metabolic Uncouplers on the Biodegradation Rate of Toluene in a Biofilm Bio-Filter Reactor
Biological inhibition of air pollution has vast advantages over physicochemical methods. One of the biggest challenges faced by researchers with traditional bio-filter in controlling Volatile Organic Compounds (VOCs) such as Benzene, Toluene, Ethylbenzene, and Xylene (BTEX) is, low degradation rate (elimination capacity) and accumulation of very high biomass. The use of metabolic uncouplers...
متن کامل